Biochemical analysis showed that i-RNA contains 22% of adenine nucleotides, 12% of uridyl and 26% of guanyl.

| September 18, 2021 | education

Biochemical analysis showed that i-RNA contains 22% of adenine nucleotides, 12% of uridyl and 26% of guanyl. Determine the nucleotide composition of the DNA from which this m-RNA was transcribed.

1) Find the amount of cytosine in the mRNA.

100% – 22% – 12% – 26% = 40%.

2) mRNA is built complementary to one of the DNA strands. This means that this chain contains 22% thymine, 12% adenine, 26% cytosine and 40% guanine.

The second chain will contain 22% adenine, 12% thymine, 26% guanine and 40% cytosine.

3) Find the content in the double chain of each of the bases. We take the total amount of nitrogen bases in both chains as 200%, then:
the amount of adenine = (12 + 22)% / 200% = 17%, the same amount of thymine according to Chargaff’s rule.

The amount of guanine = (40 + 26)% / 200% = 22%, the same amount of cytosine.



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