What factors can determine the rate of enzymatic reactions?

The activity of enzymatic reactions depends on environmental conditions.

An increase in temperature increases kinetic energy. In a liquid, this means that a more random collision of molecules occurs per unit time. Since enzymes catalyze reactions by accidentally colliding with substrate molecules, increasing the temperature increases the reaction rate, producing more product.

Any change in pH above or below the optimum can quickly lead to a decrease in the reaction rate.

Changes in enzyme and substrate concentrations affect the reaction rate of an enzyme-catalyzed reaction.